Abcam, ab251280, Anti-PMF-1 antibody [EPR17298] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PMF-1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17298,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251280 is the carrier-free version of
ab199531
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PMF-1 also recognized as Polyamine-modulated factor 1 is a protein with a molecular mass of around 63 kDa. It plays a mechanical role in transcriptional regulation by interacting with other proteins and DNA sequences. PMF-1 is expressed mainly in the nucleus of cells across various tissues. It partners with other transcription factors modulating gene expression essential for cellular growth and differentiation. This interaction underlines its importance in transcriptional control.
Biological function summary
PMF-1 engages in modifying transcriptional responses by forming part of the TCF/LEF transcription factor complex. This complex influences the expression of genes involved in cellular proliferation and differentiation. PMF-1 functions by binding to specific DNA sequences enhancing or repressing the transcription of target genes. Through these actions it serves as a regulatory hub for controlling numerous cellular responses including but not limited to cell cycle progression and apoptosis.
Pathways
PMF-1 interacts significantly within the Wnt signaling pathway. This pathway is pivotal for regulating cellular processes related to development and growth. PMF-1 associates with proteins such as β-catenin facilitating the transcriptional activation of Wnt target genes. Through this pathway PMF-1 helps transmit signals that dictate cellular fate decisions illustrating its importance in maintaining physiological balance.
PMF-1 has notable implications in cancer and neurodegenerative conditions. In cancer its association with the Wnt signaling pathway implies roles in cell proliferation and tumor progression. The interaction with β-catenin further solidifies its connection to oncogenic processes. In neurodegenerative disorders altered PMF-1 activity can influence neuronal growth and survival implicating it in pathologies like Alzheimer's disease through dysregulated transcriptional responses.