Abcam, ab251306, Anti-STAT5a+STAT5b antibody [EPR16668] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal STAT5b antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16668,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251306 is the carrier-free version of
ab200341
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The targets STAT5a and STAT5b also known as Signal Transducer and Activator of Transcription 5A and 5B form part of the STAT family and play essential mechanical roles in cellular signaling. Most commonly these proteins exhibit a molecular mass of approximately 90 kDa each. They are widely expressed in various tissues including the liver muscle and kidney as well as in different immune cells. STAT5a and STAT5b mediate signal transduction by getting phosphorylated dimerizing and translocating to the cell nucleus where they regulate the transcription of specific genes.
Biological function summary
These targets influence many cellular functions and contribute to cell proliferation differentiation and apoptosis. STAT5a and STAT5b often act as part of a larger transcriptional complex that binds to DNA and activates gene expression. In the immune system these proteins help modulate responses by regulating cytokine production. Their activity also extends to mammary gland development where they regulate milk production genes and they have roles in hematopoiesis affecting the development of blood cell types.
Pathways
The functions of STAT5a and STAT5b integrate mainly within the JAK-STAT signaling pathway which is pivotal for relaying cytokine signals from the cell membrane to the nucleus. This pathway links directly to proteins such as Janus kinases (JAKs) that phosphorylate STAT5 initiating its activation. Another key pathway is the PI3K-Akt signaling pathway where STAT5 can interact with components like mTOR to regulate cell growth and survival. These pathways are central to maintaining cellular homeostasis and immune function.
STAT5a and STAT5b have significant connections to cancer and immune-related disorders. Abnormal STAT5 signaling often due to mutations or aberrant expression is linked to various hematological malignancies including leukemias. In these conditions STAT5 partners with oncogenic proteins like BCR-ABL leading to unchecked cell proliferation. Furthermore defective STAT5 signaling can contribute to immune dysregulation disorders where connections to proteins like IL-2R are important for normal immune response. Understanding these links opens avenues for targeted therapies that aim to modulate STAT5 activity in disease contexts.