Abcam, ab251411, Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CYFIP1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17848-87,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251411 is the carrier-free version of
ab204129
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The proteins CYFIP2 and CYFIP1 also known as cytoplasmic FMR1-interacting proteins 2 and 1 are mechanistically involved in actin cytoskeleton regulation. Their molecular weights are approximately 147 kDa for CYFIP2 and 140 kDa for CYFIP1. Both proteins are widely expressed across various tissues with notable expression in the brain and other neural tissues. CYFIP2 and CYFIP1 interact with the WAVE regulatory complex influencing actin dynamics and in turn cellular morphology and movement.
Biological function summary
These proteins function by serving as scaffolds in cellular processes impacting synaptic plasticity and neuronal function. CYFIP1 and CYFIP2 are integral members of the WAVE regulatory complex which is important for inducing actin polymerization. By associating with FMRP (Fragile X Mental Retardation Protein) CYFIP proteins help modulate mRNA translation linked to synaptic development. This involvement in synaptic modulation highlights their role in the nervous system's regulatory mechanisms.
Pathways
Both CYFIP1 and CYFIP2 play key roles in the RAC1/CDC42 signaling pathway critical for actin filament organization. They interact with proteins such as WAVE Arp2/3 complex and FMRP linking them to processes that control cell shape and motility. This connection to the RAC1/CDC42 pathway and their association with the FMRP complex highlight their involvement in cytoskeleton reorganization and mRNA transport.
CYFIP2 and CYFIP1 have associations with neurological conditions like Fragile X syndrome and autism spectrum disorders. These proteins through their interaction with FMRP influence synaptic connectivity and function indicating their potential role in these diseases. Additionally CYFIP1 dysregulation has been linked to schizophrenia possibly through disruptions in actin cytoskeleton signaling and related synaptic processes.