Abcam, ab251433, Anti-FXYD3 antibody [EPR17160] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FXYD3 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17160,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251433 is the carrier-free version of
ab205534
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FXYD3 also known as Mat-8 plays a role as a regulatory subunit associated with the Na+/K+-ATPase pump modulating its ion transport activity. The protein has a molecular weight of approximately 10 kDa. FXYD3 is expressed in various tissues but it shows higher levels in epithelial tissues particularly in gastrointestinal and mammary tissues. The protein consists of a single transmembrane domain which suggests its function in modulating ion exchange across membranes.
Biological function summary
FXYD3 modulates ion transport processes important for maintaining cellular homeostasis. It forms part of the larger Na+/K+-ATPase complex interacting with other subunits to influence the pump's activity. This association affects the electrochemical gradients across the cell membrane impacting various cellular processes including volume regulation pH balance and nutrient uptake. Therefore FXYD3 is involved in key physiological roles in tissues where electrolyte balance is essential.
Pathways
FXYD3 integrates into ion transport and signaling pathways including the sodium ion transmembrane transport pathway. It interacts closely with proteins like the α and β subunits of the Na+/K+-ATPase in this context regulating their pump activity. This integration with the sodium ion transmembrane transport and signal transduction pathways exemplifies FXYD3’s role in cellular ion homeostasis and how it may influence specific physiological responses.
FXYD3 shows links to cancer particularly breast cancer. Altered expression of FXYD3 has been observed in malignant tissues suggesting a contribution to tumor development and progression. The relationship between FXYD3 and proteins like E-cadherin in this context may indicate its role in cellular adhesion and migration alterations seen in cancer. Another disorder linked to FXYD3 is cystic fibrosis where its modulation of ion channels could affect disease severity or progression especially regarding electrolyte transport abnormalities.