Abcam, ab251874, Anti-RNF138/HSD-4 antibody

Size: 100µL
Rabbit Polyclonal RNF138/HSD-4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RNF138 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RNF138 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.Q8WVD3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF138 also known as HSD-4 or RING finger protein 138 acts as an E3 ubiquitin-protein ligase. It influences protein degradation by attaching ubiquitin to target proteins signaling them for proteasomal degradation. This protein typically has a molecular mass of approximately 28 kDa. RNF138 is broadly expressed in various tissues but often shows higher expression in neuronal tissues and skin. The presence of a RING finger domain enables RNF138 to interact with substrates and E2 conjugating enzymes directly.
Biological function summary
The function of RNF138 plays a significant role in DNA damage repair and neuronal development. It often operates within a larger protein complex collaborating with other RING finger proteins and enzymes involved in ubiquitination. By facilitating the removal of damaged or excess proteins RNF138 supports cellular homeostasis. Its specific activity in neurons suggests a vital involvement in neural differentiation and axonogenesis processes essential for healthy nervous system development.
Pathways
Multiple cellular networks integrate RNF138 function including the ubiquitin-proteasome pathway and the DNA damage response pathway. It interacts with key proteins like BRCA1 and RAD51 modulating their stability and function. The involvement of RNF138 in these pathways highlights its role in maintaining genetic stability and regulating cellular responses to stress. It ensures proper cellular function by influencing protein turnover rates important for cell cycle and repair mechanisms.
Researchers associate RNF138 dysfunction with neurological disorders and certain types of cancers. Aberrant expression or mutation in RNF138 can lead to improper ubiquitination processes contributing to tumorigenesis linked with proteins like p53 and MDM2. In neurodegenerative diseases its altered activity might affect neuron survival due to disrupted protein homeostasis. Understanding RNF138 interactions provides potential therapeutic targets for mitigating these disorders' impact.