Abcam, ab25189, Biotin Anti-Integrin alpha 5 antibody [5H10-27]

Size: 100µg
Rat Monoclonal Integrin alpha 5 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing ITGA5 protein.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:5H10-27,
Isotype:IgG2a,
Light chain type:kappa,
Conjugation:Biotin,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing ITGA5 protein. The exact immunogen used to generate this antibody is proprietary information.P08648,
Specificity:ab25189 recognises alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.

Product details:
The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Integrin alpha 5 also known as CD49e or ITGA5 is a subunit of the integrin family that forms heterodimers with beta 1 integrin. It has an approximate mass of 130 kDa. This protein mainly expresses in various tissues including fibroblasts endothelial cells and certain epithelial cells. Integrin alpha 5 mediates cell adhesion by binding to fibronectin an important extracellular matrix protein helping cells stay attached to their surrounding environment.
Biological function summary
Integrin alpha 5 plays a significant role in cellular processes such as migration proliferation and differentiation. It pairs with beta 1 integrin to form the alpha 5 beta 1 integrin complex. This complex facilitates the transmission of mechanical and chemical signals between the extracellular matrix and the cell interior. Integrin alpha 5 also interacts with intracellular proteins like talin and kindlin which are essential for initiating and strengthening the linkage to the actin cytoskeleton.
Pathways
Integrin alpha 5 is involved in the regulation of several critical signaling pathways. It prominently features within the MAPK/ERK pathway and the focal adhesion signaling pathway. Through these pathways integrin alpha 5 influences cell survival proliferation and migration. It interacts with proteins such as paxillin and focal adhesion kinase (FAK) within these pathways contributing to cellular responses to external stimuli.
Integrin alpha 5 is linked to conditions such as cancer and fibrosis. Overexpression or aberrant regulation of this integrin often associates with tumorigenesis particularly in breast and colorectal cancers enhancing cancer cell migration and invasion. Integrin alpha 5 is also connected to proteins like matrix metalloproteinases (MMPs) which play roles in extracellular matrix remodeling and are involved in fibrotic diseases. These associations highlight the importance of integrin alpha 5 in pathological processes and its potential as a target for therapeutic intervention.