Abcam, ab252190, Mismatch Repair (MSH6, PMS2, MLH1, MSH2) Antibody Panel - Human

Size: 1Kit
Mismatch Repair (MSH6, PMS2, MLH1, MSH2) Antibody Panel - Human (ab252190) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
Reacts with:Human,
Target:MLH1See target data

Product details:
Mismatch Repair (MSH6, PMS2, MLH1, MSH2) Antibody Panel - Human ab252190 contains multiple trial-sized versions of anti-human antibody clones against MSH6, PMS2, MLH1, and MSH2, specifically selected for their high performance in multiple applications including IHC. This panel contains 4 recombinant rabbit monoclonal antibodies that are all knock-out validated to ensure specificity to their targets. They are provided as a sampler panel to allow you to easily evaluate each in your required application.
DNA mismatch repair (MMR) proteins are involved in repairing mistakes that occur during DNA replication and recombination, in addition to repairing some types of DNA damage. Defects in the MMR process due to mutations in MMR genes (MSH6, PMS2, MLH1, MSH2) can result in microsatellite instability (MSI), where a DNA sequence accumulates errors and produces abnormally long or shorter microsatellites. These defects in the MMR pathway have been linked to various human cancers, such as human non-polyposis colon cancer (HNPCC) and Muir-Torre Syndrome (MTS), a subtype of HNPCC.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR3894] to MLH1 (20 μL)
ab92312
- Rabbit monoclonal [EPR21017-123] to MSH2 (20 μL)
ab227941
- Rabbit monoclonal [EPR3945] to MSH6 (20 μL)
ab92471
- Rabbit monoclonal [EPR3947] to PMS2 (20 μL)
ab110638
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The proteins MSH2 PMS2 MLH1 and MSH6 play significant roles in the mismatch repair (MMR) pathway. MSH2 also known as MutS Homolog 2 has a molecular mass of approximately 100 kDa and commonly pairs with MSH6 to form a complex. These proteins are expressed in various tissues and are critical components for recognizing and repairing mismatches during DNA replication. These components are essential for maintaining genomic stability and preventing mutations from accumulating.
Biological function summary
MSH2 and related proteins assemble into essential heterodimeric complexes for the mismatch repair system. MSH2 pairs with MSH6 to form the MutSα complex while MLH1 partners with PMS2 to create the MutLα complex. Together these complexes identify and initiate repair of DNA base mismatches that can arise during replication. This precise operation ensures that the DNA's faithful transmission occurs from one generation of cells to the next highlighting the importance of maintaining integrity in diseases where genome instability is a factor.
Pathways
These mismatch repair proteins are pivotal in the cell cycle control and DNA damage response pathways. The MMR pathway is closely associated with the p53 pathway which detects damage and can promote apoptosis if the mutation burden is high. MSH2 plays an important role in recognizing mutation-inducing errors and it directly interacts with other proteins like MLH1 to maintain this checkpoint. These pathways collectively guard the cell against tumorigenesis by facilitating accurate DNA repair or triggering cell death in the face of irreparable damage.
Defects in mismatch repair proteins including MSH2 correlate strongly with Lynch syndrome also known as hereditary nonpolyposis colorectal cancer (HNPCC). This condition arises due to inherited mutations that impair the MMR system. In this context MSH2 mutations frequently co-occur with MLH1 mutations significantly elevating the risk of colorectal and endometrial cancers. Determining the status of MMR proteins using immunohistochemistry (IHC) such as the MMR IHC panel assists in diagnosing these disorders and guiding therapeutic decisions.