Abcam, ab252254, Anti-BACH2 antibody [7A4] - BSA and Azide free

Size: 100µg / 1mg
Rat Recombinant Monoclonal BACH2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:7A4,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab252254 is the carrier-free version of
ab243148
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BACH2 also known as BTB and CNC homology 2 is a transcription factor with a molecular mass of around 95 kDa. It regulates the expression of certain genes by binding to specific DNA sequences. BACH2 maintains a zinc-finger domain and a BTB domain which are important for its binding and transcriptional repressor functions. The target expresses predominantly in immune cells especially in B cells and T cells playing a critical role in immune system regulation. It is also found in other tissues but its levels are comparatively higher in lymphoid organs.
Biological function summary
BACH2 plays an important role in the regulation of immune responses. It functions as a homodimer or forms heterodimers with other proteins such as BACH1 allowing it to modulate gene expression effectively. BACH2 regulates the balance between immune tolerance and activation by controlling the production of cytokines and the differentiation of T cells. This regulation is important for preventing autoimmune responses while enabling proper immune responses against infections.
Pathways
BACH2 participates actively in the oxidative stress response and pathways of lymphocyte development. In the oxidative stress pathway it interacts with proteins like KEAP1 and NRF2 to regulate antioxidant gene expression. In lymphocyte development it influences the recombination of immunoglobulin genes and supports the maturation of B cells into plasma cells. By doing so BACH2 ensures the proper functioning of the immune system working in conjunction with various transcriptional regulatory networks.
BACH2 has associations with several immune-related conditions. It is implicated in autoimmune diseases such as type 1 diabetes where improper regulation may lead to immune system attacks on healthy cells. Additionally changes in BACH2 expression levels have been linked to lymphoma a type of blood cancer involving the lymphatic system. BACH2 works closely with proteins like BCL6 and PRDM1 in these contexts influencing cell proliferation and survival pathways which can impact both tumor development and autoimmune pathology.