Abcam, ab25235, Anti-CD16+CD32 antibody [93] - BSA and Azide free

Size: 100µg
Rat Monoclonal FCGR3 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, BL and reacts with Mouse, Human samples. Cited in 21 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:93,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:ICC/IF, BL, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:ab25235 reacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.

Product details:
This product was changed from ascites to tissue culture supernatant on 02/08/2019. Lot numbers higher than GR3269503 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD16 and CD32 also referred to as FCγRIII and FCγRII are proteins expressed on the surface of various immune cells like natural killer cells macrophages and some subsets of T cells. CD16 has a mass ranging between 50-80 kDa while CD32 exhibits variability in size between 32-40 kDa due to different isoforms. These proteins play pivotal roles in immune response by binding to the Fc region of immunoglobulins influencing cell activation and signaling.
Biological function summary
CD16 and CD32 serve important roles in mediating immune cell interactions. They work as part of immune synapses facilitating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis processes. Due to their involvement in these complex activities they are included in immunological synapse formation and modulation extending their influence over overall immune regulation and response. These proteins are known to contribute to both innate and adaptive immune mechanisms as receptors for the Fc portion of immunoglobulin G.
Pathways
CD16 and CD32 interact within signaling pathways like the Fc gamma receptor-mediated phagocytosis pathway and the immune response-regulating signaling pathway. These receptors work in coordination with other immunoglobulin family members to initiate targeted cellular reactions necessary for immune challenge responses. CD32 often partners with CD19 and CD21 within pathways to modulate B cell receptor-mediated signaling.
CD16 and CD32 have connections to autoimmune diseases and infectious diseases. Aberrant expression or dysfunction in these proteins can contribute to the development of conditions such as systemic lupus erythematosus where improper immune complex clearance occurs and chronic inflammation results. CD16's involvement with NK cells can also play a role in viral infection control highlighting their importance in pathogen defense mechanisms.