Abcam, ab2524, Anti-Blood Group B Antigen antibody [HEB-29]

Size: 1mL
Mouse Monoclonal ABO antibody. Carrier free. Suitable for Agg, IHC-P and reacts with Human samples. Cited in 8 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:HEB-29,
Isotype:IgM,
Light chain type:unknown,
Carrier free:Yes,
Reacts with:Human,
Applications:Agg, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody recognizes human blood group B antigens. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined., Storage buffer-Constituents: Tissue culture supernatant, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Blood Group B Antigen commonly called the B antigen is a carbohydrate antigen found on the surface of red blood cells. This antigen is part of the ABO blood group system which determines an individual's blood type. The B antigen's molecular composition consists primarily of a sugar molecule called D-galactose. The B antigen is expressed on the erythrocyte surface and is encoded by the B allele of the ABO gene. Individuals with blood type B will have the B antigen on the surface of their red blood cells and do not have anti-B antibodies in their plasma. The mass of the B antigen can vary depending on the glycoproteins and glycolipids it attaches to on the membrane.
Biological function summary
The B antigen's presence on red blood cells plays a fundamental role in immune system recognition and compatibility during blood transfusions. It is not part of a complex but associates directly with the cell membrane structures. The presence of particular carbohydrate sequences such as those of the B antigen allows the immune system to distinguish between self and non-self cells. Known as anti-B antibodies these antibodies exist in individuals who do not express the B antigen specifically those with blood type A or O.
Pathways
Blood Group B Antigen contributes significantly to the ABO blood group system's immunological pathway. This pathway includes biosynthesis and degradation processes involving the glycosyltransferase enzyme responsible for adding sugar residues to the precursor substance to form specific antigens. Glycosyltransferase is related to protein pathways that handle energy metabolism and cellular processes due to its enzymatic role in forming cell surface markers like the B antigen.
The B antigen is linked to transfusion reactions and hemolytic disease of the newborn (HDN). Incompatibility between donor and recipient blood types during transfusions can cause immunological reactions where anti-B antibodies attack the B antigens. These reactions can lead to severe complications. HDN occurs when a mother with type O blood carries a baby with type B blood resulting in anti-B antibodies attacking fetal red blood cells. This emphasizes the clinical significance of the B antigen in blood type compatibility and transfusion medicine.