Abcam, ab252403, Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free

Size: 100µg / 1mg
Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403) is a rabbit recombinant monoclonal antibody for Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3915,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:We recommend not to boil the samples after lysis to get desired WB bands.

Product details:
Product Specifications
Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403) is a recombinant rabbit monoclonal antibody which was developed by Abcam using patented rabbit monoclonal antibody technology. ab252403 is provided in a carrier-free, conjugation ready format, and is validated in Flow Cyt (Intra), ICC/IF, IHC-P and WB in human, mouse and rat samples. Carrier-free antibodies are compatible with our best-in-class range of Lightning-Link® conjugation kits.
Quality and Validation
The specificity of Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403) has been confirmed by testing in knockout samples.
Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403) has 6 independent reviews from customers.
Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403) specifically detects Glucose Transporter GLUT1 (UniProt ID: P11166; Molecular weight: 54kDa) and is sold in 100 ug and 1mg selling sizes.
Related Products
Antibody clone EPR3915 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, HRP, Alexa Fluor® 488, Alexa Fluor® 594, PE, Alexa Fluor® 405, APC, FITC (
ab195020
ab195021
ab195359
ab206360
ab209449
ab210438
ab316298
ab322306
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
Biological function summary
This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.
Pathways
GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.
GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.