Abcam, ab252692, Anti-Hepatitis B Surface Antigen (Adw) antibody [B3228M]

Size: 50µg
Mouse Monoclonal Hepatitis B Surface Antigen (Adw) antibody. Carrier free. Suitable for ELISA, WB, ICC/IF, IM and reacts with Hepatitis B virus samples. Cited in 2 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:B3228M,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Hepatitis B virus,
Applications:WB, IM, ELISA, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:HBsAg pre-S2 antigen (amino acids 132-137, sequence 'AGGSSS') (adw nomenclature). Reference start codon was Pre-S1. Epitope recognition confirmed by competing with peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from TCS., Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Hepatitis B Surface Antigen (Adw) often called 'HBsAg Adw' or just 'Adw' serves as a marker for the hepatitis B virus (HBV) infection. It is a structural protein with an approximate mass of 24 kDa. It prominently expresses on the surface of HBV and infectious particles in the bloodstream of infected individuals. This antigen is key in serological diagnosis and monitoring of HBV providing an indicator of viral presence and replication status in the host.
Biological function summary
The Hepatitis B Surface Antigen plays a central role in the life cycle of the virus. It participates in the formation of the viral envelope an essential step for virion assembly and release. Additionally it associates with other proteins as part of a nucleocapsid complex that encapsulates and protects the viral DNA within the host organism. This interaction facilitates immune evasion and allows persistent infection making HBsAg an important factor in chronic hepatitis B infection.
Pathways
The surface antigen of HBV integrates itself into the viral replication and host immune response pathways. HBsAg impacts the host immune regulation by modulating pathways that normally detect and clear foreign antigens. It interferes with cytokine signaling pathways which include interactions with proteins like interferons that serve as antiviral agents. These protein interactions hamper the host's ability to mount an effective immune response fostering a suitable environment for viral persistence.
Hepatitis B Surface Antigen is intimately connected to hepatic diseases such as hepatitis B infection and hepatocellular carcinoma (HCC). Persistent expression of HBsAg in patients indicates chronic infection significantly increasing the risk of liver damage and cancer development. Liver fibrosis and cirrhosis are also linked to HBsAg often observed in tandem with proteins like core antigen HBcAg which exacerbates inflammation and cellular damage. This relationship underlines HBsAg's importance in the pathogenesis and progression of HBV-related liver diseases.