Abcam, ab252707, Anti-Dengue Virus 1+2+3+4 antibody [BDI419]

Size: 250µg
Mouse Monoclonal Dengue Virus 1 antibody. Suitable for ELISA, Dot, IF and reacts with Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4 samples. Immunogen corresponding to Virus preparation containing Dengue Virus 1 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:BDI419,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Dengue virus 4, Dengue virus 3, Dengue virus 2, Dengue virus 1,
Applications:ELISA, IF, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.Virus preparation containing Dengue Virus 2 protein.The exact immunogen used to generate this antibody is proprietary information.Virus preparation containing Dengue virus 3 protein.The exact immunogen used to generate this antibody is proprietary information.Virus preparation containing Dengue virus 4 protein.,
Specificity:The reactivity of this antibody varies between strains and various isolates, therefore, a single dilution may not be appropriate for each situation. The myeloma cell line used as a fusion partner produces small amounts of IgG1, therefore IgG1 is sometimes present in the IgG2a, k preparation.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from TCS., Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Dengue Virus commonly known as DENV includes four serotypes: DENV-1 DENV-2 DENV-3 and DENV-4. Each serotype comprises a complex structure with a viral envelope containing the structural proteins envelope (E) and membrane (M) among others. The envelope protein is key for entry into host cells by mediating binding and fusion. The non-structural proteins such as NS3 and NS5 are involved in viral replication and assembly. The virus circulates across tropical and subtropical regions primarily transmitted by Aedes aegypti mosquitoes.
Biological function summary
Dengue Virus impacts human cells by initiating a series of interactions once it enters the blood system. It hijacks the host's cellular machinery to replicate and generate viral particles. It triggers immune responses leading to production of antibodies such as anti-Dengue antibodies. The virus does not operate alone but within a complex involving the host immune response influencing cell signaling pathways.
Pathways
Dengue Virus is integrated into the type I interferon signaling pathway and the TLR3 signaling pathway. These pathways trigger antiviral responses that attempt to limit viral replication. The virus interacts with host proteins including STAT1 and IRF3 which play roles in these pathways. The virus evades the immune response by modifying these pathways enhancing its survival and replication in the host.
Dengue Virus is connected to Dengue Fever and Dengue Hemorrhagic Fever which can lead to severe complications and even death. It modulates host proteins involving cytokines like TNF-alpha and interleukins contributing to pathogenesis and immune system breakdown. Understanding the virus’s interaction with these proteins is essential in the development of vaccines and therapeutic antibodies targeting Dengue infections.