Abcam, ab253236, Anti-West Nile Virus NS1 antibody [IC12.E7.G9/01]

Size: 100µg
Mouse Monoclonal POLG antibody. Suitable for ELISA and reacts with West Nile virus samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:IC12.E7.G9/01,
Isotype:IgG1,
Carrier free:No,
Reacts with:West Nile virus,
Applications:ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody is specific for the NS1 protein of West Nile virus, and does not cross-react with NS1 from other flaviviruses, including Dengue virus serotypes 1- 4, Zika virus, Yellow Fever virus and Japanese Encephalitis virus. No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-pH: 7 - 8Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The West Nile Virus NS1 protein is a nonstructural protein with a molecular weight of approximately 46 kDa. This protein is often referred to simply as NS1 and it plays an important role in the viral replication process. It is expressed both intracellularly and extracellularly during infection with the West Nile Virus. Intracellularly it functions in the replication complex within the host cell while extracellularly it is secreted as a hexamer.
Biological function summary
The NS1 protein aids in immune evasion by interfering with the host's complement system. This protein forms a homodimer in the endoplasmic reticulum which is part of its functional form in the host cell. It also interacts with other viral proteins to form complexes that facilitate viral replication. NS1 modulates the immune response hampering the host's ability to mount an effective defense against the virus.
Pathways
The NS1 protein is part of the viral replication and assembly pathway in infected cells. It interacts with other nonstructural proteins such as NS4A and NS4B which are part of the replication complex. NS1 assists in creating an environment favorable for the virus by altering host cell processes and pathways ensuring efficient viral genome replication and assembly.
The NS1 protein has a significant association with West Nile Fever and neuroinvasive disease. It contributes to the pathogenesis of these diseases by disrupting the host's immune response which allows the virus to persist and cause more severe symptoms. The NS1 protein has connections with inflammatory proteins and complement components which it binds to in order to avoid immune elimination playing an essential role in the severity of the disease presentations.