Abcam, ab253237, Anti-Yellow Fever Virus NS1 antibody [HH7.B.D10.B8]

Size: 100µg
Mouse Monoclonal POLG antibody. Carrier free. Suitable for ELISA and reacts with Yellow fever virus samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:HH7.B.D10.B8,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Yellow fever virus,
Applications:ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody is specific for the NS1 protein of Yellow Fever virus, and does not cross-react with NS1 from other flaviviruses, including Dengue virus serotypes 1- 4, Zika virus, West Nile virus and Japanese Encephalitis virus. No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-pH: 7 - 8Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Yellow Fever Virus NS1 also known as Non-structural Protein 1 plays an important mechanical role in the viral lifecycle of yellow fever virus. This protein is approximately 46 kDa in size and is mainly expressed within the cytoplasm and secreted outside of infected host cells. NS1 assists in various aspects of virus replication and immune evasion particularly through its involvement in the formation of the viral replication complex. The secreted form of NS1 gets actively released from infected cells contributing to immune system modulation.
Biological function summary
The Non-structural Protein 1 promotes viral infection and survival. It facilitates viral replication by contributing to the formation of the replication organelle within the host cell. NS1 also interacts with host immune components hindering effective immune response. Although it is not part of a larger viral complex NS1 works synergistically with other non-structural proteins of yellow fever virus such as NS3 to enhance replication efficiency and immune evasion capabilities.
Pathways
Yellow Fever Virus NS1 is deeply implicated in viral replication and immune evasion mechanisms. In the viral replication pathway it aids the virus in establishing replication sites and stabilizing the viral replication machinery. Its interaction with the host immune signaling pathway interferes with the activation of the host immune response enabling the virus to persist. NS1 is intricately linked to other proteins such as NS4B in the viral replication machinery and indirectly supports immune evasion through these pathways.
Yellow Fever Virus NS1 contributes significantly to the pathogenicity of yellow fever a serious viral hemorrhagic disease. The protein plays a role in the development of the severe systemic symptoms associated with yellow fever infection by evading host immune detection which leads to liver damage and multi-organ failure. NS1 may influence the virulence of the virus by modulating the host's immune response and its interaction with host proteins can exacerbate disease outcomes. Understanding the mechanisms of NS1 could inform strategies for therapeutics and vaccine development for yellow fever.