Abcam, ab253689, Human HIV1 p24 Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human HIV1 p24 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human HIV1 p24.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:15.63 - 1000 pg/mL,
Assay Platform:Reagents

Product details:
Human HIV1 p24 Antibody Pair is a matched pair of unconjugated
recombinant rabbit monoclonal
capture and detection antibodies used to quantify Human HIV1 p24 in sandwich ELISAs and many other pair-based applications.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIV1 p24 also known as HIV p24 protein is a core protein of the Human Immunodeficiency Virus type 1 (HIV-1). The p24 protein has a molecular weight of about 24 kDa. HIV1 p24 comprises part of the viral capsid which encases the viral RNA genome. This protein is prominently expressed during the early stages of HIV infection. The presence of HIV1 p24 is commonly detected through various laboratory techniques including the use of p24 ELISA kits.
Biological function summary
HIV1 p24 plays an important role in the viral life cycle. It assists in the formation and stability of the viral capsid which is critical for maintaining the integrity of the viral core. HIV p24 is not a lone actor; it is part of the structural framework and interacts with other viral proteins to facilitate the viral assembly and maturation processes. The detection of HIV1 p24 is a marker for viral replication and infection stage.
Pathways
The function of HIV1 p24 aligns significantly within the HIV replication pathway and the host’s immune response pathway. HIV p24 is intrinsically tied to processes of viral assembly alongside proteins like Gag and Pol coordinating to ensure successful viral replication. Furthermore the immune system recognizes the HIV1 p24 protein thereby integrating it into the host immune response which is important in disease progression and for diagnostic purposes like in the HIV ELISA assay.
HIV1 p24 is integrally connected to HIV/AIDS a disorder that profoundly affects the immune system. The presence of HIV p24 in the bloodstream is an early marker of infection and is frequently monitored to track the disease's progression. Alterations in the levels of p24 protein relate to the efficiency of therapeutic interventions. The study of HIV1 p24 also brings focus on its interaction with the CD4 receptor which is pivotal in the viral entry process further linking various stages of the HIV infection cycle to potential therapeutic targets.