Abcam, ab253730, Human 14-3-3 zeta (YWHAZ) Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human 14-3-3 zeta (YWHAZ) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human 14-3-3 zeta (YWHAZ).
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:250 - 8000 pg/mL,
Assay Platform:Reagents

Product details:
Human 14-3-3 zeta (YWHAZ) Antibody Pair is a matched pair of unconjugated
recombinant rabbit monoclonal
capture and detection antibodies used to quantify Human 14-3-3 zeta in sandwich ELISAs and many other pair-based applications.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 14-3-3 proteins are a family of regulatory molecules with a molecular mass around 28 to 32 kDa. These proteins exist in seven isoforms in humans termed beta gamma epsilon zeta eta theta and sigma. 14-3-3 proteins are ubiquitously expressed in eukaryotic cells including neurons liver cells and immune cells. These proteins participate in modulation of many cellular processes by binding to phosphorylated serine and threonine residues on various target proteins. This binding alters the conformation of target proteins influencing their activity subcellular localization or stability.
Biological function summary
14-3-3 proteins are involved in signal transduction cell cycle control and apoptosis regulation. They participate in protein interactions and form dimers that serve as scaffolds assembling complexes and linking signaling pathways. The proteins associate with kinases such as Raf-1 thereby contributing to signaling pathways that include MAPK/ERK. At the cellular level 14-3-3 proteins govern key mechanisms like the G1/S phase transition and stress response which are critical for maintaining cellular homeostasis.
Pathways
14-3-3 proteins play critical roles in the PI3K/AKT and MAPK/ERK pathways. They interact with several key proteins within these pathways including AKT and Raf-1 mediating cellular responses to growth factors and stress. In the PI3K/AKT pathway 14-3-3 proteins help regulate the progression of the cell cycle and promote cell survival linking extracellular signals to the machinery that governs cell fate. These interactions underline the role of 14-3-3 proteins in integrating and regulating complex cell signaling networks.
14-3-3 proteins are associated with neurodegenerative diseases like Alzheimer's and certain types of cancer. In Alzheimer's disease 14-3-3 proteins interact with tau protein contributing to the pathological aggregation of tau associated with neuronal damage. Similarly in various cancers their interaction with cell cycle regulators like p53 can influence tumor progression by affecting cell division and apoptosis. These connections highlight the involvement of 14-3-3 proteins in disease mechanisms making them potential targets for therapeutic intervention.