Abcam, ab253845, Human PLTP Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human PLTP Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human PLTP.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Assay Platform:Reagents

Product details:
Human PLTP is a matched pair of unconjugated
recombinant rabbit monoclonal
capture and detection antibodies used to quantify Human PLTP in sandwich ELISAs and many other pair-based applications.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PLTP also known as Phospholipid Transfer Protein is an important component in human lipid metabolism. This protein functions by transferring phospholipids between different types of lipoproteins in plasma. PLTP possesses a molecular mass of about 56 kDa. Its expression occurs mainly in the liver and adipose tissue with additional presence in the brain and lungs. These locales of expression facilitate its key roles in lipid processing and lipoprotein metabolism.
Biological function summary
PLTP plays a significant role in remodeling high-density lipoproteins (HDL) and regulating lipoprotein particles. PLTP exists as a part of a larger complex involved in these processes interacting closely with other proteins in the lipoprotein maturation and transformation. This activity of PLTP significantly affects the levels and sizes of HDL particles in circulation thereby influencing cholesterol homeostasis within the body.
Pathways
PLTP acts within the reverse cholesterol transport pathway important for the removal of excess cholesterol from peripheral tissues. The protein works alongside Apolipoprotein A-I (ApoA-I) and Lecithin-Cholesterol Acyltransferase (LCAT) to promote cholesterol efflux and HDL maturation. Another key pathway involving PLTP is the lipid metabolism pathway where it influences lipid levels and impacts the function of other proteins such as Apolipoprotein E (ApoE).
PLTP has associations with atherosclerosis and metabolic syndrome. Variations in PLTP activity or expression can lead to altered HDL levels increasing the risk for heart disease and related cardiovascular conditions. In atherosclerosis PLTP's relationship with ApoA-I becomes especially relevant as this interaction influences lipid transport dynamics and plaque formation. Additionally in metabolic syndrome abnormal PLTP function may contribute to dyslipidemia a common factor in metabolic disorders.