Abcam, ab254013, Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human

Size: 1Kit
Macrophage Marker panel containing 5 antibodies against Human CD11b, CD68, CD163, CD14, CD16 - Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Key facts
Reacts with:Human,
Target:ITGAMSee target data

Product details:
Product Specifications
Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013) is a panel of 6 rabbit recombinant monoclonal antibodies to macrophage markers. Macrophage markers are proteins used to identify and study macrophages, immune cells involved in phagocytosis, inflammation and tissue repair.
This panel contains antibodies to CD11b (UniProt: P11215), CD68 (UniProt: P34810), CD163 (UniProt: Q2VLH6), CD14 (UniProt: P08571) and CD16 (UniProt: P08637) in convenient trial sizes (20 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.
The individual antibodies included in this panel are:
Rabbit recombinant monoclonal anti-CD11b antibody [EP1345Y] - C-terminal (
ab52478
Rabbit recombinant monoclonal anti-CD68 antibody [EPR20545] (
ab213363
Rabbit recombinant monoclonal anti-CD163 antibody [EPR19518] (
ab182422
Rabbit recombinant monoclonal anti-CD14 antibody [SP192] (
ab183322
Rabbit recombinant monoclonal anti-CD16 antibody [SP175] (
ab183354
All of these component antibodies are available for purchase individually using their corresponding abIDs.
Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
All antibodies in this panel have a minimum of 50 citations in peer-reviewed journals and are trusted by scientific community.
Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD11b CD14 CD16 CD68 and CD163 are important macrophage markers. CD11b also known as integrin alpha M has a mass of about 170 kDa is found on the surface of macrophages neutrophils and some T cells. It plays a mechanical role in cell adhesion migration and phagocytosis. CD14 acts as a co-receptor for the detection of bacterial lipopolysaccharides and is expressed on monocytes and macrophages. CD16 or FcγRIII is involved in antibody-dependent cellular cytotoxicity (ADCC) found on natural killer cells macrophages and some granulocytes. CD68 a marker of macrophage lineage is important in phagocytosis and is present in tissue macrophages. CD163 known for scavenging hemoglobin-haptoglobin complexes is expressed on monocytes and macrophages aiding in anti-inflammatory responses.
Biological function summary
These macrophage markers participate in essential immune system functions. CD11b binds to iC3b opsonized particles facilitating phagocytosis and inflammatory signaling. CD14 acting with TLR4 triggers innate immune responses against pathogens. CD16 engages with the Fc region of IgG antibodies mediating ADCC. CD68 is part of the lysosomal/endosomal membrane associating with scavenger receptors in phagocytosis and tissue remodeling. CD163 modulates anti-inflammatory processes by binding to hemoglobin-haptoglobin complexes leading to the release of anti-inflammatory cytokines. These markers are vital to immune surveillance and the clearance of pathogens and debris.
Pathways
CD11b and CD14 play roles in the Toll-like receptor (TLR) signaling pathway which is critical for initiating immune responses. CD11b interacts with complement component iC3b aiding in immune complex clearance. CD16 is involved in the FcγRIIB-mediated inhibitory pathway balancing immune activation. CD68 contributes to pathways involved in lysosomal degradation processes. CD163 is linked to the IL-10-mediated anti-inflammatory signaling pathway. These macrophage markers contribute to the coordination of host defense and inflammatory resolution.
CD11b is associated with chronic inflammatory diseases such as rheumatoid arthritis due to its role in leukocyte recruitment. CD14 has connections to sepsis as it participates in the recognition of bacterial LPS leading to systemic inflammation. CD16 contributes to autoimmune diseases; its dysfunction can lead to impaired ADCC. CD68 levels have been studied in atherosclerosis linking it to foam cell formation and plaque stability. CD163 with its anti-inflammatory properties relates to inflammatory disorders like multiple sclerosis linked to hemoglobin-haptoglobin clearance and IL-10 production. These markers offer potential therapeutic targets for treating these diseases.