Abcam, ab254080, Anti-Nuclei antibody [235-1]

Size: 100µL
Mouse Monoclonal Human Nuclei antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Human Nuclei protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:235-1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Specific for endogenous levels of human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band.

Product details:
This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The nuclei serve as the command center of eukaryotic cells housing the genetic material in the form of DNA and its associated proteins. Commonly known as the cell's "control center" the nucleus typically weighs in at approximately 1.8 x 10^12 Daltons when considering a moderate-sized nucleus though exact mass can vary based on cell type. Nuclei reside within the cytoplasm of cells delineated by the double-layered nuclear envelope. Within it chromatin (which comprises DNA wrapped around histone proteins) and the nucleolus (site of ribosomal RNA synthesis) are key components.
Biological function summary
The nucleus plays a central role in governing cell activities through the regulation of gene expression. It controls transcription processes and executes RNA processing tasks. It is part of several macromolecular complexes including chromatin-remodeling complexes and splicing machinery. These complexes are important for efficient transcriptional regulation and RNA maturation enabling precise cellular responses to environmental and developmental cues.
Pathways
Nuclei integrate information from signaling pathways to modulate gene expression. They are vital components of pathways such as the cell cycle regulation pathway and the DNA damage response. They coordinate with proteins like cyclins and cyclin-dependent kinases (CDKs) in cell cycle progression and with proteins such as p53 and BRCA1/2 to repair DNA damage and prevent genomic instability. These pathways ensure proper cell division and stability of genetic information across generations.
Dysfunction in the nuclei can have serious implications for human health including cancer and neurodegenerative conditions. Mutations or dysregulations in nuclear proteins and DNA repair mechanisms such as those involving p53 or lamin proteins can lead to genomic instability and the development of various cancers. In neurodegenerative disorders nuclear abnormalities affecting RNA transport and processing may contribute to disease pathology highlighting the importance of nuclear integrity in maintaining neuronal function.