Abcam, ab254731, Anti-NLE1/nle antibody

Size: 100µL
Rabbit Polyclonal NLE1/nle antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NLE1 aa 250-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NLE1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.Q9NVX2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NLE1 also known as Notchless homolog 1 is a core protein involved in ribosomal biogenesis and maturation. This protein gets expressed in various tissue types with higher occurrence in actively dividing cells such as in the bone marrow. The exact molecular mass of NLE1 is approximately 78 kDa. NLE1's primary mechanical function involves ensuring the proper assembly and functioning of the ribosomal subunits during protein synthesis.
Biological function summary
The Notchless homolog 1 protein plays a critical role in the regulation of ribosome assembly. NLE1 is a part of the ribosome assembly complex and facilitates the maturation of 60S ribosomal subunit which is important for efficient protein synthesis. By doing so it directly influences cell growth and proliferation. NLE1 can regulate multiple cellular processes by impacting protein synthesis rates within cells.
Pathways
Ribosome biogenesis and Notch signaling involve significant activities by NLE1 in cellular contexts. NLE1 participates in the ribosomal biogenesis pathway important for cell proliferation and growth. Additionally connections with the Notch signaling pathway place NLE1 in interaction with proteins like NOTCH1 which influences cell fate determinations and differentiation events in tissues.
Improper function of NLE1 associates with conditions related to abnormal cell proliferation such as cancer. Elevated levels of NLE1 expression have been observed in several cancer types indicating a potential role in oncogenesis. Furthermore the involvement of NLE1 in certain malignancies has been connected to altered interactions with proteins like NOTCH1 making the signaling and modulation of these pathways a critical area of research in unraveling cancer mechanisms.