Abcam, ab254774, Anti-Espin antibody

Size: 100µL
Rabbit Polyclonal Espin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ESPN aa 750-850.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ESPN aa 750-850. The exact immunogen used to generate this antibody is proprietary information.B1AK53

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Espin also known as actin-bundling protein is a protein important for the development of hair cell stereocilia in cochlea. It has a molecular mass of approximately 116 kDa. Espin is mainly expressed in cells that have hair-like projections which include hair cells of the inner ear and in the Sertoli cells in testis. These cells rely on Espin to maintain their structure and function playing a pivotal role in stabilizing the parallel actin filament bundles essential for cell projections' rigidity and strength.
Biological function summary
Espin is essential for actin cytoskeleton organization. It acts as an important component in forming cross-links between actin filaments enhancing their stability and organization. Espin is not generally considered part of a complex but it directly interacts with actin in cells. The protein influences cellular processes like cell shape alterations and sensory cell function by regulating actin assembly dynamics. This ability to control actin filaments directly impacts how sensory inputs like sound vibration are relayed and processed.
Pathways
Espin plays a substantial role in the actin cytoskeleton reorganization pathway which is integral to maintaining cellular architecture and mechanotransduction. Espin associates with other actin-binding proteins such as fascin and fimbrin which also contribute to actin bundling and stabilization. Additionally Espin's interactions with these proteins support stereocilia formation and maintenance critical for auditory and balance functions in mammals.
Espin mutations have shown connections to disorders like Non-syndromic Deafness and Usher Syndrome. In Non-syndromic Deafness Espin dysfunction can lead to impaired stereocilia development resulting in hearing loss. Similarly mutations affecting Espin are linked with Usher Syndrome where hearing and vision are disrupted often with Espin's interactions with protocadherin proteins being implicated. Understanding these associations highlights the importance of Espin's proper function in sensory health and stress the potential impact of its dysregulation in related disorders.