Abcam, ab254811, Anti-ENGASE antibody

Size: 100µL
Rabbit Polyclonal ENGASE antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ENGASE aa 50-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ENGASE aa 50-200. The exact immunogen used to generate this antibody is proprietary information.Q8NFI3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ENGASE protein also known as Endo-β-N-acetylglucosaminidase catalyzes the hydrolysis of N-linked glycans from glycoproteins or free oligosaccharides in the cytosol. This enzyme has a molecular mass of approximately 37 kDa. ENGASE is expressed in various tissues with substantial expression in liver and kidney tissues. Its enzymatic activity serves in the degradation and recycling of glycoproteins and is important for maintaining cellular homeostasis.
Biological function summary
The ENGASE enzyme participates in the processing of glycoprotein degradation via the lysosomal pathway. It functions as a monomer rather than part of a larger complex. By playing a role in the removal of glycan chains ENGASE contributes to the energy supply and cell maintenance through its involvement in the salvage pathways of glycoprotein turnover. Its activity complements that of other enzymes in glycan catabolism highlighting its importance in cell biology.
Pathways
This enzyme is an integral part of glycoprotein catabolic processes and directly links to the N-glycan biosynthesis pathway. It works alongside lysosomal enzymes including glycosidases and glucosidases to ensure the complete breakdown of glycoproteins and manage cellular cleaning processes. ENGASE interacts with proteins like Man2A1 and GanAB which aid in further modifications and processing of glycan moieties ensuring proper cellular function and vitality.
ENGASE has been associated with lysosomal storage disorders such as Gaucher's disease where glycoprotein degradation is impaired. In this context the lack or malfunction of ENGASE enzyme activity can lead to accumulation of undegraded glycoproteins which might play a contributory role in pathogenesis. Additionally in certain cancers where glycosylation patterns are altered ENGASE's activity can be dysregulated potentially affecting tumor progression or response to therapy. In these diseases ENGASE interacts with proteins like glucocerebrosidase which also play roles in glycan degradation and metabolism.