Abcam, ab254853, Anti-RFC5 antibody

Size: 100µL
Rabbit Polyclonal RFC5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RFC5 aa 150-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RFC5 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.P40937

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RFC5 also known as Replication Factor C subunit 5 or RFC38 is a critical component of the RFC complex involved in DNA replication and repair. This protein has a molecular mass of approximately 38 kDa. It is expressed in various tissues serving an essential function in maintaining genomic stability. The RFC5 protein associates with other subunits to form a complex that acts as a clamp loader during DNA replication.
Biological function summary
RFC5 plays an important role in coordinating the proper functioning of the DNA replication machinery. It is part of the larger RFC complex which comprises five subunits working in concert to load the proliferating cell nuclear antigen (PCNA) onto DNA strands. This loading action is fundamental to the process of DNA synthesis ensuring that DNA polymerases can operate effectively. By collaborating with other components of the replication machinery RFC5 ensures accuracy and efficiency during cell division.
Pathways
RFC5 participates in DNA replication and repair pathways. It is closely linked with the DNA damage response pathway where it associates with the PCNA and other replication factors such as polymerase δ and polymerase ε. Through these interactions RFC5 helps initiate and sustain the replication fork stability and repair mechanisms important for genomic integrity.
RFC5 associates with conditions like cancer and genomic instability disorders. Mutations or dysregulation of RFC5 may contribute to uncontrolled cell proliferation in cancers. Its interaction with the PCNA and other replication factors highlights its relevance in tumorigenesis and genomic maintenance. Studying RFC5 can provide insights into potential therapeutic targets for cancers associated with replication stress and DNA repair defects.