Abcam, ab25547, PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

Size: 100µg
Mouse Monoclonal HA11 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:27-11-13,
Isotype:IgG2a,
Light chain type:kappa,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:This antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.,
Specificity:ab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.3Preservative: 0.09% Sodium azideConstituents: PBS, 16% Sucrose, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MHC Class I H2-D1 also known as H2-Db is an important protein within the major histocompatibility complex. This protein carries a molecular mass of approximately 45 kDa. H2-Db is expressed on the surface of almost all nucleated cells. As a protein it plays an important role in immune response by presenting peptide antigens to cytotoxic T cells. Other similar proteins like H2-Kb and H2-Kd are also involved in antigen presentation though each has distinct peptide binding preferences.
Biological function summary
The MHC Class I H2-Db interacts with peptides derived from cytosolic proteins and loads them onto its structure. It forms a complex with beta-2 microglobulin and presents these peptides on the cell surface. This action is essential in signaling to CD8+ T cells which scan for non-self peptides originating from infections or cellular abnormalities. The method by which H2-Db binds peptides ensures precise immune surveillance.
Pathways
H2-Db operates prominently in the antigen processing and presentation pathway. This pathway plays an important role in immune response and involves proteases that generate peptide fragments. H2-Db interacts with transporter associated with antigen processing (TAP) proteins to bind peptides enabling effective display of antigens. Furthermore its activity connects with other immune proteins such as H2-Kb in presenting a comprehensive range of antigens to immune cells.
The involvement of H2-Db in autoimmune diseases and viral infections is significant. Certain autoimmune conditions arise when self-antigens are mistakenly presented triggering an immune response. Infections like viral hepatitis show how pathogens can manipulate H2-Db to evade immune detection. Tumor cells may also exploit H2-Db's antigen-presenting function. Proteins such as H2-Kb may interact similarly highlighting the need for MHC Class I diversity in combating disease.