Abcam, ab255841, Anti-PAX1 antibody [5A2] - BSA and Azide free

Size: 100µg / 1mg
Rat Recombinant Monoclonal PAX1 antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 2 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:5A2,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Mouse,
Applications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab255841 is the carrier-free version of
ab252847
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PAX1 also known as paired box protein Pax-1 is a transcription factor with a mass of approximately 52 kDa. This protein features a paired domain that mediates DNA binding and plays a significant role in transcriptional regulation. PAX1 is expressed in the notochord presomitic mesoderm and connective tissues during embryonic development. It is important during pattern formation and particularly important in vertebral column and skeletal development.
Biological function summary
The paired box protein PAX1 functions as an important player in embryogenesis primarily influencing the formation and differentiation of tissues. It is not known to be part of any large protein complex. PAX1 regulates the expression of various genes involved in the development of the axial skeleton particularly in the vertebral column. The accurate expression of PAX1 ensures proper skeletal structure formation acting as a determinant in vertebrate development.
Pathways
PAX1 contributes significantly to the Hedgehog and Wnt signaling pathways both of which are indispensable in regulating cell growth differentiation and patterning during embryogenesis. PAX1 collaborates with other proteins like Gli3 an important transcription factor in Hedgehog signaling. Within these pathways PAX1 ensures proper signaling cascades that streamline developmental processes and morphology.
Alteration or mutation in the PAX1 gene associates with vertebral malformations and disorders such as scoliosis and Klippel-Feil syndrome. Studies indicate that disruptions in PAX1 expression can lead to these congenital abnormalities by affecting the protein's regulatory function in skeletal development. The protein Barx1 which also plays a role in skeletal development can show interconnected interactions with PAX1 highlighting the complexity of genetic regulation in these disorders.