Abcam, ab255963, Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal hair cortex Cytokeratin/K40 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AE13,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Rat, Mouse,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab255963 is the carrier-free version of
ab289545
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hair cortex Cytokeratin also known as K40 is a type of intermediate filament protein with a molecular mass of approximately 40 kDa. It is an important structural component in the hair shaft particularly within the cortex where it contributes to the mechanical properties of hair fiber. K40 is predominantly expressed in keratinocytes found in the hair follicle's inner layers including the scalp. This protein provides toughness and elasticity to the hair and it plays a significant role in maintaining hair integrity.
Biological function summary
Cytokeratin K40 helps form a stable cytoskeletal network that supports the structural framework of the hair shaft. Within the keratin family K40 is part of a larger complex of keratin proteins that interact to form strong fibrous structures. It partners with other keratins like AE13 to reinforce the mechanical durability of hair. This complex network supports the rigidity and resilience necessary to resist environmental damage and mechanical stress.
Pathways
The function of hair cortex Cytokeratin K40 interweaves with processes involved in epithelial cell differentiation and hair formation. K40 aligns with the keratinization pathway where it collaborates with related keratins to implement the transformation of keratinocytes into the keratin-rich hair cortex. This pathway connects K40 with proteins like AE1 and AE13 which together facilitate the assembly of a robust cytoskeletal matrix essential for forming healthy hair.
Alterations in Cytokeratin K40 expression can relate to hair shaft disorders and conditions like alopecia. These diseases often arise when the normal architecture and strength of hair fibers are compromised. Within this context changes in K40 may also impact the activity of other keratin proteins such as b and AE13 exacerbating the severity of hair brittleness and growth issues. Understanding the role of K40 may help develop therapeutic strategies to manage or treat associated hair disorders.