Abcam, ab256361, Anti-DNA:RNA hybrid antibody [S9.6] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal DNA:RNA hybrid antibody. Carrier free. Suitable for IP, Dot and reacts with Nucleic Acid samples. Cited in 4 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:S9.6,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Applications:IP, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The S9.6 monoclonal recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA (PubMed IDs: 2422282, 16614443). The antibody has high affinity for DNA-RNA hybrids but also binds RNA-RNA hybrids that are AU-rich (PubMed ID: 23784994). The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since R-loop sequence affects binding affinity (PubMed ID: 28594954). It is important to use controls such as RNase A treatment in light of the cross-reactivity with double stranded RNA. Please see our DNA-RNA-IP (DRIP) protocol for further information. We do not recommend using this antibody for imaging applications such as ICC-IF, IHC or Flow due to the cross-reactivity with double stranded RNA.

Product details:
The pCALM3_2 vector used to generate R-loops was kindly provided by Prof. Frederic Chedin at UC Davis. The pCALM3_2 plasmid carries a 789bp fragment of the human CALM3 region that forms R-loops (PubMed ID: 31053798) cloned between T3 and T7 RNA polymerase promoter sequences. Transcription by T3 RNA polymerase leads to R-loop formation. Transcription by T7 RNA polymerase does not allow R-loop formation.
Our DNA-RNA immunoprecipitation (DRIP) protocol is linked
ab256361 is the carrier-free version of
ab234957
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze