Abcam, ab25681, Anti-MHC class II I E kappa antibody [14-4-4S]

Size: 250µg
Mouse Monoclonal HB2J antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Tissue preparation containing MHC class II I E kappa protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:14-4-4S,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific to an epitope on mouse I-E kappa MHC class II alloantigen. The antibody reacts with the I-E kappa class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to cross react with the rat class II alloantigen RT1D.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 8.2Constituents: 100% Borate buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MHC class II I-E kappa also known as I-Ek is a protein involved in the immune system's antigen-presenting function. It is part of the major histocompatibility complex (MHC) class II molecules which are important for presenting exogenous antigens to CD4+ T helper cells. MHC class II molecules have a molecular mass that ranges from 50 to 70 kDa. I-Ek is usually expressed on the surface of professional antigen-presenting cells like dendritic cells macrophages and B cells.
Biological function summary
I-Ek is involved in the immune response by forming a complex with peptides derived from external sources. This complex is then presented on the cell surface to facilitate recognition by CD4+ T helper cells which play a critical role in adaptive immunity. I-Ek's interactions are essential for triggering T cell-mediated immune responses important in fighting infections and maintaining immune tolerance. Researchers widely study this protein to understand immune response modulation.
Pathways
MHC class II I-Ek is a component of the antigen processing and presentation pathway critical in adaptive immune responses. Within this pathway the I-Ek protein interacts with the invariant chain and memory B cells to assist in antigen presentation. It also participates in the intricate network where it communicates with CD4+ receptor-bearing T cells important for effective immune system activation.
MHC class II I-Ek has significant implications in autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. Aberrant expression or malfunction of I-Ek can contribute to these conditions by promoting inappropriate immune responses against self-antigens. The protein interacts with other molecules like human leukocyte antigen (HLA) linked to autoimmunity serving as potential research targets for therapeutic intervention.