Abcam, ab257517, Human MAP3K11 (MLK3) knockout HeLa cell lysate

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MAP3K11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 1 bp deletion in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 1 bp deletion in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MAP3K11, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MLK3 also known as MEKK11 is a kinase widely expressed in human tissues. This protein with a molecular mass of approximately 97 kDa functions as a mitogen-activated protein kinase kinase kinase (MAP3K). MLK3 mechanically activates the c-Jun N-terminal kinase (JNK) and p38 MAP kinase pathways by phosphorylating downstream MAP2Ks. It is involved in various cell processes including apoptosis and inflammation.
Biological function summary
MLK3 plays a critical role in cellular response to stress and inflammation. It acts as part of a signaling complex that regulates the JNK and p38 pathways key mediators in immune responses. These pathways control the production of pro-inflammatory cytokines and the cellular stress response. Through its kinase activity MLK3 supports the activation of transcription factors such as AP-1 which drive gene expression changes needed for its biological functions.
Pathways
MLK3 is intricately involved in the MAPK signaling cascade. It connects with other MAP3Ks like MLK1 and MLK2 contributing to the complexity of MAPK signaling. MLK3’s participation in the JNK and p38 pathways links it to cytokine signaling and cellular stress responses. These pathways are significant for maintaining cellular homeostasis and coordinating responses to external stimuli.
MLK3 has implications in cancer and neurodegenerative diseases. In cancer MLK3 modulates pathways that influence cell proliferation and survival potentially leading to tumor growth and metastasis when dysregulated. Similarly altered MLK3 signaling is observed in neurodegenerative diseases possibly due to its role in managing cellular stress. MLK3 interacts with proteins like JNK and p38 which are important in disease mechanisms and progression.