Abcam, ab257540, Human MPP5 (Pals1) knockout HeLa cell lysate

Size: 1Kit
PALS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PALS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MPP5 also known as Pals1 is a membrane-associated guanylate kinase (MAGUK) protein. It has a molecular weight of approximately 75 kDa. MPP5 is expressed in various tissues with significant levels in the brain retina and epithelial cells. MPP5 is important in cell junction organization ensuring proper cellular architecture and polarity. It occupies a role as a scaffolding protein forming large protein complexes at cell membranes.
Biological function summary
MPP5 is part of the Crumbs protein complex which includes Crb3 and PatJ proteins. This complex is essential for maintaining apical-basal polarity in epithelial cells. MPP5 contributes to cell polarity and adhesion ensuring integrity and proper functioning of epithelial tissue. Disruptions in this protein complex can lead to loss of cell polarity affecting tissue organization and stability.
Pathways
MPP5 interacts with proteins in the Hippo signaling pathway which controls organ size and cell growth. MPP5 influences the activity of YAP (Yes-associated protein) a critical component in the Hippo pathway. YAP regulation by MPP5 can affect cellular proliferation and apoptosis linking MPP5 to tissue growth and regeneration processes.
Mutations or dysregulation in MPP5 have connections to retinal degeneration and epithelial cancers. Its interaction with proteins like CRB1 in the retina influences photoreceptor development and retinal maintenance. Abnormal expression of MPP5 can lead to disrupted cell polarity contributing to cancer progression and tumor metastasis in epithelial tissues. Understanding MPP5's role in these diseases helps in identifying potential therapeutic targets.