Abcam, ab258035, Human LTB4R2 (Leukotriene B4 Receptor 2) knockout HeLa cell lysate

Size: 1Kit
LTB4R2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 22 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 22 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LTB4R2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Leukotriene B4 Receptor 2 (BLT2) also known as LTB4R2 plays an important mechanical role as a G-protein coupled receptor (GPCR) that binds leukotriene B4 (LTB4) a pro-inflammatory lipid mediator. BLT2 has a molecular mass of approximately 41 kDa and exhibits expression notably in tissues such as the spleen liver and intestinal tract. Alternative names for this receptor include BLTR2 or GPR43. It functions as part of the rapid cellular response in these tissues during immune reactions.
Biological function summary
The receptor BLT2 significantly contributes to the regulation of inflammatory responses. This receptor does not form part of a functional complex but operates independently to mediate chemotaxis and aggregation of immune cells like neutrophils. By interacting with leukotriene B4 BLT2 facilitates diverse immune responses including leukocyte recruitment and activation. Such activities underline its importance in acute inflammatory reactions and pathogen defense mechanisms.
Pathways
BLT2 influences and integrates into key cellular signaling routes such as the MAPK/ERK and PI3K/Akt pathways. These pathways affect cellular processes like proliferation survival and differentiation. BLT2's interaction with proteins involved in these pathways like ERK (Extracellular Signal-Regulated Kinases) or Akt (protein kinase B) demonstrate its role in amplifying and regulating immune responses during prolonged inflammation.
BLT2 has associations with inflammatory diseases including asthma and rheumatoid arthritis. These conditions involve chronic inflammation where BLT2 together with proteins like cytokines and other chemoattractant receptors exacerbates cellular recruitment and inflammatory signaling. By linking hyperactive immune cell responses BLT2 serves as a potential target in moderating disease manifestations and developing anti-inflammatory leukotriene-based therapies.