Abcam, ab258219, Human SYNJ2 (Synaptojanin 2) knockout HeLa cell lysate

Size: 1Kit
SYNJ2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon3 and 68 bp deletion in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon3 and 68 bp deletion in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SYNJ2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Synaptojanin 2 also known as SYNJ2 is a protein encoded by the SYNJ2 gene. It has a molecular mass of approximately 180 kDa. Synaptojanin 2 functions as a phosphoinositide phosphatase which helps dephosphorylate phosphatidylinositol 45-bisphosphate (PIP2) to phosphatidylinositol (PI). This affects the membrane dynamics and signal transduction. The expression of synaptojanin 2 occurs in various tissues with notable abundance in the brain and testis.
Biological function summary
Synaptojanin 2 plays an important role in synaptic vesicle trafficking and endocytosis. It can function as a part of protein complexes involved in these processes. The protein is involved in rearranging the actin cytoskeleton which impacts cellular shape and movement. Its activity influences synaptic transmission efficiency by controlling the cycle of synaptic vesicles particularly in the nervous system which reflects its high expression in the brain.
Pathways
Synaptojanin 2 is closely linked to the phosphatidylinositol signaling pathway and the endocytosis pathway. These pathways are important for cellular communication and nutrient uptake. Within these pathways synaptojanin 2 interacts with proteins like dynamin a GTPase that mediates membrane fission during endocytosis. Synaptojanin 2 also functions in relation to AP-2 a clathrin adaptor complex that plays a role in vesicle formation highlighting its participation in endocytic trafficking.
Synaptojanin 2 relates to conditions such as cancer and neurodegenerative diseases. Overexpression or mutation of synaptojanin 2 associates with certain types of cancers including breast cancer potentially via disrupted signal transduction. It maintains a connection with proteins like PTEN a known tumor suppressor through its involvement in lipid signaling. In neurodegenerative diseases abnormal synaptojanin 2 function may impact neuronal health and is linked to diseases such as Alzheimer's disease. Defective phosphoinositide metabolism influences amyloid precursor protein processing implicating synaptojanin 2 in the pathology of these disorders.