Abcam, ab258228, Human TDRD7 knockout A549 cell lysate

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TDRD7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 2 bp deletion in exon8 and 95 bp insertion in exon8.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 2 bp deletion in exon8 and 95 bp insertion in exon8.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TDRD7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TDRD7 also known as Tudor Domain Containing 7 is a protein involved in RNA processing and regulation. It has a molecular weight of approximately 130 kilodaltons. Researchers have identified its expression largely in the testes eyes and brain. TDRD7 belongs to the Tudor domain-containing protein family and plays an important role in maintaining cellular RNA dynamics by interacting with various molecules in RNA-related processes.
Biological function summary
TDRD7 contributes to the formation and function of ribonucleoprotein (RNP) complexes needed for germ cell development and lens maturation. It actively participates in RNA granule assembly important for RNA stabilization and localization in cells. Through its Tudor domains TDRD7 binds symmetrically dimethylated arginines on other proteins recognizing and organizing these RNP complexes effectively.
Pathways
TDRD7 integrates into RNA regulatory pathways influencing processes such as mRNA splicing and degradation. It interacts with proteins like PIWIL1 and PIWIL2 which are involved in the piRNA pathway important for maintaining genomic integrity in germ cells. TDRD7 helps mediate gene expression regulation through these pathways impacting cellular functions and organismal development.
Mutations or dysregulation of TDRD7 are associated with congenital cataracts and other lens-related diseases. Moreover it has connections with gonadal development disorders due to its critical function in the testes. Disruptions in TDRD7 can link with proteins such as CRYAA and CRYBB2 important in maintaining the transparency and refractive properties of the lens leading to cataract formation when altered.