Abcam, ab258236, Human TMOD3 (Tropomodulin 3) knockout HeLa cell lysate

Size: 1Kit
TMOD3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and 8 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and 8 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMOD3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tropomodulin 3 also known as TMOD3 serves an important role in regulating the dynamics of the actin cytoskeleton. It caps the pointed end of actin filaments which prevents both their elongation and disassembly. TMOD3 has a molecular mass of approximately 40 kDa. This protein is expressed in various tissues including the heart liver and lungs indicating its broad functional significance across multiple systems.
Biological function summary
Tropomodulin 3 interacts with components of the cytoskeleton to help maintain cell shape and stability. It often functions as part of a multi-protein complex with tropomyosin which stabilizes the actin filament and protects it from depolymerization. Through its interaction with actin and tropomyosin TMOD3 influences cell motility division and muscle contraction essential processes in cellular and tissue dynamics.
Pathways
Tropomodulin 3 plays a part in the actin cytoskeleton regulation pathway. It regulates the turnover and length of actin filaments which connect to processes like cell adhesion and movement. The regulation of actin filament dynamics by TMOD3 influences interactions with associated proteins such as myosin which drives cellular movements and mechanical support.
Tropomodulin 3 has been linked to specific pathologies such as cardiomyopathies and certain cancers. In conditions of cardiomyopathy TMOD3 expression or function disturbances can lead to improper cardiac muscle function often involving related proteins like myosin and tropomyosin. Moreover aberrant TMOD3 activity has also been associated with cancer metastasis where altered actin dynamics contribute to increased cell invasiveness and tumor spread.