Abcam, ab258266, Human UBE2E1 (UBCH6) knockout HEK-293T cell lysate

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UBE2E1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 1 and 5 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 1 and 5 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UBE2E1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBCH6 also known as UBE2E1 is a ubiquitin-conjugating enzyme with approximately 23 kDa molecular weight. It functions mechanically by transferring ubiquitin from an E1 enzyme to a substrate protein often requiring an E3 ligase to provide specificity. This action is an important step in the ubiquitination process which tags proteins for various fates such as degradation localization or activity modulation. UBCH6 expresses ubiquitously across many tissues with noticeable levels in the liver heart and kidney indicating its broad functional role in the human body.
Biological function summary
UBCH6 participates in the regulatory control of protein homeostasis. It acts as part of the ubiquitination system which involves post-translational modifications critical for maintaining cellular functions. UBCH6 often partners with various E3 ligases to ensure proper protein turnover and response to cellular stress. It ensures the selective degradation of misfolded or damaged proteins which is essential for preventing toxic accumulation within the cell. Through these interactions UBCH6 maintains protein quality control ensuring cellular health and function.
Pathways
UBCH6 involves itself in the ubiquitin-proteasome pathway and influences the NF-kB signaling pathway. The ubiquitin-proteasome pathway regulates protein degradation impacting numerous cellular processes such as cell cycle DNA repair and apoptosis. In these pathways UBCH6 interacts with related proteins such as ubiquilin and various E3 components like the cullin-RING ligases which aid its role in cellular regulation. Specifically in NF-kB signaling UBCH6 assists in modulating inflammatory responses and innate immune responses linking cellular stress to transcriptional changes.
UBCH6's functions connect to cancer and neurodegenerative diseases. Dysregulation in its activity or expression can lead to aberrant protein degradation pathways contributing to oncogenic transformations or impaired cell cycle control as seen in specific cancers. The association extends to proteins like p53 or IκB which are important in these conditions. Additionally in neurodegenerative disorders like Parkinson's disease UBCH6's interaction with proteins involved in protein aggregation or clearance like Parkin demonstrates its role in disease pathology highlighting its importance in maintaining neuronal health and preventing disease-related protein accumulations.