Abcam, ab258268, Human UBXN1 knockout HEK-293T cell lysate

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UBXN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UBXN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBXN1 also known as UBX domain-containing protein 1 has a major role in regulating protein degradation through the ubiquitin-proteasome system. This protein weighs approximately 38 kDa. UBXN1 is expressed ubiquitously with higher levels reported in tissues such as kidney liver and brain. It interacts with the p97/VCP ATPase modulating its activity in protein degradation. By binding to ubiquitin chains UBXN1 influences the fate of many substrates targeted for degradation.
Biological function summary
UBXN1 serves as a regulator in processes such as protein homeostasis and stress response. It functions as part of a large protein complex that includes elements like p97/VCP which are central to maintaining cellular protein balance. UBXN1 modulates processes involving endoplasmic reticulum-associated degradation (ERAD). It therefore plays a role in the quality control of misfolded proteins preventing their accumulation and potential toxicity in cells.
Pathways
UBXN1 is closely associated with the ubiquitin-proteasome pathway a critical system for protein turnover. It acts along with proteins such as p97/VCP and works in tandem with ubiquitin enzymes to regulate protein substrates. Additionally UBXN1 has a role in autophagy-related pathways where it assists in removing damaged proteins and organelles cooperating with signalling proteins like LC3 which are essential for autophagic vesicle formation.
UBXN1 has connections with neurodegenerative diseases and cancer. Its involvement in protein homeostasis and degradation pathways links UBXN1 to neurodegenerative conditions where mismanagement of protein quality control is a hallmark. The dysregulation of UBXN1 alongside proteins p97/VCP has been associated with inclusion body myopathy and other degenerative diseases. Moreover alterations in UBXN1 expression or function relate to cancer progression as its imbalance can lead to uncontrolled cell growth through disrupted protein degradation mechanisms.