Abcam, ab258287, Human WNK2 knockout HEK-293T cell lysate

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WNK2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon4.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon4.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WNK2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WNK2 also known as With No Lysine (K) Kinase 2 is a serine/threonine kinase weighing approximately 250 kDa. It plays a critical role in regulating ion transport by modulating various ion channels and transporters. WNK2 is expressed in several tissues with higher expression in the central nervous system and some epithelial tissues. This kinase operates through interacting with other proteins and enzymes to facilitate its functional role in cellular processes.
Biological function summary
WNK2 contributes to cellular signaling and volume regulation. It does not form part of a complex itself but its function gets integrated through its interaction with pathways affecting cytoskeleton dynamics and cell survival. WNK2’s phosphorylation activity helps in reorganizing cellular components and aids cells in adapting to osmotic stress therefore supporting cellular homeostasis.
Pathways
Several interacting molecules highlight the significance of WNK2 in signal transduction. WNK2’s role involves the MAPK/ERK pathway where it cooperates with STE20 and other kinases to relay signals from the cell surface to the nucleus. This interaction influences cell proliferation and differentiation shedding light on WNK2's contribution to the regulation of growth signals. Another important pathway involving WNK2 is the regulation of sodium and potassium balance through its communication with related kinases like WNK1 and WNK4.
WNK2’s malfunction has links to conditions like hypertension and cancer. Abnormalities in WNK2 expression or activity can lead to altered cellular homeostasis and excessive cell proliferation which get commonly observed in various types of cancer. Dysregulation in WNK2 activity also relates to hypertension where it affects sodium and water retention partly through interaction with kinases such as WNK3. Understanding these connections can prove valuable in developing interventions targeting these diseases.