Abcam, ab258500, Human LRRC58 knockout HeLa cell lysate

Size: 1Kit
LRRC58 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LRRC58, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LRRC58 also known as Leucine-rich repeat-containing protein 58 is a protein with a recognized role in cellular processes. It has a molecular mass of around 60 kDa. Researchers observed its expression in various tissues though it is highly expressed in the brain. The leucine-rich repeats within the protein suggest a role in protein-protein interactions wherein it may interact with other cellular components to facilitate specific mechanical functions.
Biological function summary
LRRC58 contributes to cellular processes through its involvement in signaling pathways. While detailed functions are under study there is evidence suggesting that it could form part of larger protein complexes influencing downstream molecular signaling. This interaction possibly regulates cellular growth and differentiation important for maintaining healthy cellular environments.
Pathways
Scientists have linked LRRC58 to the MAPK/ERK signaling pathway which is important in regulating cell division and differentiation. It may interact with proteins such as MEK1/2 and ERK1/2 in this context. Another possible relationship exists with the PI3K/AKT pathway which indicates a role in cell survival and metabolism regulation. The exact mechanism of LRRC58's involvement in these pathways is an area of active research.
LRRC58 has shown potential connections to neurological conditions particularly neurodegenerative diseases. Altered expression levels of LRRC58 could play a role in the progression of such disorders possibly interacting with proteins implicated in these conditions like Tau or Amyloid precursor protein. Further investigation is needed to clarify the interactions between LRRC58 and these proteins in pathological settings.