Abcam, ab258503, Human MAF1 knockout HEK-293T cell lysate

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MAF1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2 and 4 bp deletion in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2 and 4 bp deletion in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MAF1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAF1 also known as MAF1 homolog is a negative regulator of RNA polymerase III an important enzyme in the synthesis of small RNAs such as tRNA and 5S rRNA. The molecular mass of MAF1 is approximately 29 kDa. This protein mainly expresses in the nucleus of eukaryotic cells. MAF1's activity is controlled by phosphorylation which governs its ability to repress the transcription of RNA polymerase III.
Biological function summary
MAF1 is significant in cellular growth and metabolism regulation. It is not part of a larger protein complex but interacts with several proteins to exert its regulatory functions. MAF1 acts as a brake on RNA polymerase III activity responding to changes in cellular environmental conditions such as nutrient availability and stress. By doing so MAF1 helps coordinate cellular responses to shifts in energy levels and stress modulating the balance between cell growth and maintenance.
Pathways
MAF1 plays a role in the mTOR signaling pathway which is important for cell growth proliferation and survival. Within this pathway MAF1 functions as a downstream effector that inhibits RNA transcription when mTORC1 activity decreases. It relates closely to other proteins such as mTOR and AKT which are also involved in cellular responses to nutrient and energy fluctuations. By influencing RNA polymerase III transcription MAF1 contributes to the regulation of protein synthesis according to metabolic needs.
Alterations in MAF1 activity associate with cancer and metabolic syndromes. In cancer reduced MAF1 activity can lead to unchecked RNA polymerase III activity promoting fast cell proliferation and tumor growth. In metabolic disorders MAF1's involvement in nutrient sensing pathways links to insulin resistance when dysregulated. Through these conditions MAF1 connects to proteins like mTOR and AKT and its malfunction can exacerbate disease progression due to disrupted cell growth control.