Abcam, ab258524, Human MPST (MST) knockout HEK-293T cell lysate

Size: 1Kit
MPST KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MPST, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MST also known as Mammalian Sterile 20-like kinase or STK3 is a serine/threonine kinase with a molecular mass of about 60 kDa. MST is expressed in various tissues including the brain liver and skeletal muscle. This kinase functions mechanically by phosphorylating downstream substrates exerting control over cell proliferation apoptosis and cytoskeletal reorganization. Alternate names like 3-MST also exist which can sometimes lead to confusion regarding its precise identity among similar kinases.
Biological function summary
MST plays a significant role in the regulation of the Hippo signaling pathway particularly as a core component of the MST kinase complex. This complex interacts with several other proteins to control organ size and suppress tumor development by regulating the balance between cell division and apoptosis. MST kinases exert influence on cell cycle checkpoints and promote cellular apoptosis aiding in the maintenance of tissue homeostasis.
Pathways
MST proteins are essential within the Hippo signaling pathway and are integrally involved in modulating the MAPK pathway. MST interacts with proteins like LATS1/2 activating them to help mediate the phosphorylation of downstream effectors including YAP and TAZ in the Hippo pathway. These interactions lead to transcriptional changes that govern cell proliferation and apoptosis highlighting MST's role in ensuring proper cellular responses to external and internal stimuli.
MST dysregulation is closely linked to cancers and neurodegenerative diseases. In cancer MST's inability to regulate cell growth can result in uninhibited cell proliferation as seen in hepatocellular carcinoma where MST's interaction with YAP becomes dysfunctional. In neurodegenerative disorders particularly Alzheimer's disease pathways involving MST and related proteins such as MARK may contribute to pathological tau hyperphosphorylation leading to neuronal damage. Understanding MST's relation to these diseases aids in targeting therapeutic interventions.