Abcam, ab258552, Human OCIAD1 knockout HEK-293T cell lysate

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OCIAD1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-OCIAD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OCIAD1 also known as ovary carcinoma-associated disintegrin-domain containing protein 1 is a protein with a mass of approximately 35 kDa. It is expressed in various tissues with high levels observed in the ovaries and the central nervous system. Structurally OCIAD1 contains domains that may influence cellular interactions and signal transduction. Studies suggest that OCIAD1 influences cellular processes by participating in membrane-associated complexes which could be important for its mechanical roles.
Biological function summary
OCIAD1 engages in cellular signaling and regulation. It might be a part of a protein complex that contributes to cellular adhesion and proliferation. OCIAD1 appears to support cellular growth and may steer certain cell developmental pathways. By interacting with other proteins it could modulate cellular response mechanisms suggesting its importance in maintaining normal cellular function.
Pathways
OCIAD1 plays roles that impact the PI3K/AKT signaling pathway and the MAPK/ERK pathway. These pathways are essential for the regulation of cell survival growth and differentiation. Within these networks OCIAD1 may interact with proteins like AKT1 and ERK2 facilitating signal transduction that drives cellular responses. Its involvement in these pathways indicates a role in the modulation of critical intracellular signaling events.
OCIAD1 has an established link with ovarian cancer and neurodegenerative diseases such as Alzheimer's disease. In ovarian cancer OCIAD1 might contribute to the malignant transformation and tumor progression possibly in connection with proteins such as p53. In Alzheimer's disease altered OCIAD1 expression could affect proteins like tau which plays a significant role in neurofibrillary tangles. These associations suggest OCIAD1 as a potential target for therapeutic interventions in these conditions.