Abcam, ab258561, Human PANK4 (Pantothenate kinase 4) knockout HEK-293T cell lysate

Size: 1Kit
PANK4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PANK4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pantothenate kinase 4 also known as PANK4 plays an important role in the initial step of the coenzyme A (CoA) biosynthesis pathway. This enzyme catalyzes the phosphorylation of pantothenate (vitamin B5) to produce 4'-phosphopantothenate a critical step in the pathway. PANK4 has a mass of approximately 86 kDa. Researchers have observed its expression in various tissues including the liver which hints at its potential regulatory functions in metabolic processes across different biological systems.
Biological function summary
PANK4 participates in the regulation of CoA levels within cells impacting fatty acid metabolism and energy production. It does not exist as part of a larger protein complex but works in a network of enzymes involved in metabolic regulation. By modulating CoA availability PANK4 influences the synthesis and degradation of fatty acids playing a central role in maintaining cellular energy balance. This activity is especially important in tissues with high metabolic rates such as the liver.
Pathways
PANK4 is an important participant in the CoA biosynthesis pathway and impacts the fatty acid oxidation pathway. It functions collaboratively with other enzymes such as acyl-CoA dehydrogenases which are involved in the breakdown of fatty acids for energy production. The regulation of these pathways by PANK4 is significant for maintaining efficient energy metabolism. Its activity influences how the body stores and uses energy particularly during periods of fasting and high energy demand.
PANK4 has a connection to metabolic disorders and neurodegenerative diseases. Its role in CoA biosynthesis links it to conditions such as type 2 diabetes where improper fatty acid metabolism occurs. Additionally disruptions in CoA balance may relate PANK4 to neurodegenerative diseases like Huntington’s disease as energy dysregulation impacts neuron function. The study of PANK4 in these contexts sheds light on its potential as a therapeutic target for managing energy-related health issues.