Abcam, ab258633, Human RFX5 knockout HEK-293T cell lysate

Size: 1Kit
RFX5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RFX5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RFX5 also known as Regulatory Factor X 5 is a transcription factor characterized by a mass of approximately 74 kDa. It plays an essential role in the modulation and transcriptional regulation of genes. RFX5 is predominantly expressed in the nucleus of human cells where it binds to DNA and regulates gene expression. This protein is vital to the regulation of major histocompatibility complex (MHC) class II genes affecting how cells present antigens.
Biological function summary
RFX5 functions as part of the RFX complex an important element for the transcription of MHC class II genes. It interacts with other regulatory factor X proteins to form this complex enabling the activation of target gene transcription. RFX5 acts by binding to the X box motifs within the promoters of the MHC class II genes. Its presence is necessary to maintain the immune response capability by ensuring the expression of these genes which is a fundamental process in antigen presentation to immune cells.
Pathways
RFX5 integrates into the MHC class II antigen presentation pathway. It has a significant relationship with the CIITA transcriptional regulator with CIITA enhancing the activity of RFX5 during the assembly of the MHC class II transcriptional machinery. Another important pathway is the immune response pathway where RFX5 is important in coordinating the expression of genes that help the immune system recognize and respond to pathogens effectively.
Deficiencies or malfunctions in RFX5 have strong connections with Bare Lymphocyte Syndrome (BLS) type II marked by reduced expression of MHC class II molecules. In this context RFX5's interactions with CIITA are critical because improper function of either can lead to compromised immune responses. Studies also suggest an association between disrupted RFX5 activity and autoimmune diseases where improper antigen presentation can lead to the immune system attacking the body's own tissues.