Abcam, ab258637, Human RMDN3 (PTPIP51) knockout HeLa cell lysate

Size: 1Kit
RMDN3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp insertion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp insertion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RMDN3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PTPIP51 also known as Protein Tyrosine Phosphatase-Interacting Protein 51 functions at a mechanical level by interacting with protein tyrosine phosphatases and kinases which are key regulators of signal transduction. PTPIP51 has a molecular mass of approximately 51 kDa. The protein is expressed in various tissues including the brain testis and skeletal muscle. Its interactions suggest a role in maintaining cellular function and signaling.
Biological function summary
The PTPIP51 protein plays a role in cellular processes such as apoptosis and cellular differentiation. It is believed to associate with other proteins to form complexes which influence mitochondrial function and cellular energy metabolism. Through these interactions PTPIP51 aids in maintaining cellular homeostasis and regulating cell survival mechanisms.
Pathways
PTPIP51 participates in the regulation of the MAPK/ERK pathway and the insulin signaling pathway. It interacts with proteins such as RAF1 and GRB2 to convey signals from the cell surface to the DNA in the cell nucleus affecting cell division and proliferation. These pathways highlight the protein’s importance in controlling cellular growth and reaction to external stimuli.
PTPIP51 has connections to neurodegenerative disorders like Alzheimer's disease and metabolic conditions such as obesity. In Alzheimer’s alterations in PTPIP51 are linked with the dysregulation of mitochondrial function and it interacts with proteins such as Tau involved in neurofibrillary tangles. In obesity PTPIP51 is connected to insulin resistance engaging with protein partners like IRS1 to influence glucose uptake and metabolism.