Abcam, ab258641, Human RNF19B knockout A549 cell lysate

Size: 1Kit
RNF19B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RNF19B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF19B also known as RING finger protein 19B or Dorfin is an E3 ubiquitin-protein ligase that functions through tagging various substrates with ubiquitin marking them for degradation by the proteasome. It has a molecular weight of approximately 54 kDa. This protein appears in various tissues but shows higher expression in the brain spinal cord heart and muscle. RNF19B localizes to the cytoplasm and is known for its role in regulating several cellular processes.
Biological function summary
RNF19B participates in the endoplasmic reticulum-associated degradation (ERAD) pathway and interacts with different co-factors to form functional complexes. It facilitates the removal of misfolded or damaged proteins within the cell thereby maintaining protein homeostasis. The protein's ability to recognize and ubiquitinate misfolded proteins plays a critical part in cellular quality control mechanisms.
Pathways
RNF19B interacts significantly with the ubiquitin-proteasome system and also affects the Parkinson's disease pathway. RNF19B links to key proteins like parkin and CHIP which are involved in the degradation of proteins associated with neurodegenerative diseases. Through these interactions RNF19B modulates cell health and protein turnover highlighting its central role in maintaining cellular balance.
RNF19B exhibits a strong connection to neurodegenerative conditions like amyotrophic lateral sclerosis (ALS) and Parkinson's disease. Mutations or dysregulation of RNF19B can exacerbate cellular stress contributing to disease progression. Additionally RNF19B associates with the protein huntingtin impacting pathways related to Huntington's disease. The protein's ability to regulate misfolded protein degradation links it directly to these disease states suggesting its potential as a therapeutic target.