Abcam, ab258653, Human SDF4 knockout HEK-293T cell lysate

Size: 1Kit
SDF4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SDF4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SDF4 also known as Stromal Cell Derived Factor 4 or Cab45 is a calcium-binding protein with a molecular mass of approximately 45 kDa. It is expressed in the Golgi apparatus of cells in various tissues including pancreas liver and brain. SDF4 plays a mechanical role in sorting and trafficking of proteins through the secretory pathway specifically during the transport of secretory cargo in the Golgi.
Biological function summary
This protein participates in the regulation of calcium-dependent vesicle transport. It does not function as part of a large protein complex but interacts with different proteins in the Golgi for sorting activities. SDF4's ability to bind calcium allows it to modulate interactions necessary for vesicle movement and cargo recognition affecting how proteins exit the Golgi and are distributed to other areas of the cell.
Pathways
Studies show SDF4 is involved in the cellular secretory pathway and calcium signaling pathway. Within these pathways SDF4 interacts with proteins like co-chaperone SIL1 to ensure proper protein folding and trafficking. SDF4 also contributes to vesicle budding processes that are essential for maintaining proper cellular function and homeostasis.
Dysfunction of SDF4 links to disorders such as diabetes and neurodegenerative diseases. Abnormal SDF4 expression impacts proteins like insulin in the pancreatic pathway contributing to diabetes through misfolding or improper secretion of insulin. In neurodegenerative conditions SDF4's disrupted association with proteins involved in calcium homeostasis affects synaptic function and neuron health implicating it in diseases like Alzheimer's.