Abcam, ab258661, Human SH3BGRL3 knockout HEK-293T cell lysate

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SH3BGRL3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SH3BGRL3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SH3BGRL3 also known as SH3 domain binding glutamic acid-rich protein-like 3 is a small protein with a molecular mass of approximately 17 kDa. This protein is expressed in a variety of tissues including the liver heart and muscle. It contains an SH3 binding domain known to interact with SH3 domains of other proteins providing a regulatory mechanism in cell signaling. The protein is also highly expressed in certain tumor cells indicating its potential role in cellular transformation and cancer.
Biological function summary
The protein is involved in several cellular processes including signal transduction and cytoskeleton organization. SH3BGRL3 functions as part of a protein complex that modulates interactions between signaling molecules and cytoskeletal components. It plays an important role in maintaining cellular architecture and facilitating cell communication by interacting with other proteins inside the cell. This interaction can affect cell structure and growth.
Pathways
This protein participates in signal transduction and cytoskeletal dynamics pathways. It interacts with proteins such as Src which is involved in various signaling cascades. SH3BGRL3's connection to the Src signaling pathway is significant as it can modulate processes like cell proliferation and differentiation. The protein is also linked with actin cytoskeleton pathways further highlighting its role in maintaining cell structure integrity.
Research connects SH3BGRL3 to cancer and cardiovascular diseases. Its expression levels in tumor cells are linked to cancer progression potentially serving as a biomarker for tumor development. In cardiovascular disorders alterations in SH3BGRL3 can affect heart muscle function possibly influencing disease outcomes. The protein has associations with Src in both cancer and heart diseases suggesting a broader role in disease mechanisms through these pathways.