Abcam, ab258665, Human SIDT1 knockout A549 cell lysate

Size: 1Kit
SIDT1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon11.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon11.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SIDT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SID1 transmembrane family member 1 (SIDT1) protein is involved in the transport of small interfering RNA (siRNA) across cellular membranes. SIDT1 is also known as SID-1 homolog 1 or SID-1 transmembrane family member 1 and it has a mass of approximately 89 kDa. It is expressed in various tissues with notable expression in the placenta and kidney suggesting its functional significance in these locations.
Biological function summary
SIDT1 is involved in the RNA interference (RNAi) pathway which regulates gene expression. This protein is not known to form parts of larger protein complexes but serves as a channel for dsRNA molecules. SIDT1's activity can affect gene silencing processes by facilitating the entry of siRNA from the extracellular environment into the cell cytoplasm playing an important role in post-transcriptional gene regulation.
Pathways
SIDT1 plays a role in the RNA interference pathway important for post-transcriptional regulation of genes. It is associated with the machinery of RNAi which includes Dicer and Argonaute proteins. These interactions are important because they allow for precise gene expression control. SIDT1 facilitates the cellular uptake of siRNA thereby influencing gene expression levels and impacting regulatory pathways tied to cellular homeostasis.
SIDT1 has potential links to disorders involving aberrant gene expression such as certain carcinomas. Its role in enabling RNAi makes it a subject of interest in cancer research where dysregulated gene silencing mechanisms may contribute to disease. Connections between SIDT1 and other proteins like Argonaute in these disease processes provide avenues for therapeutic exploration making SIDT1 a target for interventions aiming to modulate RNAi-related pathways in cancer therapy.