Abcam, ab258678, Human SLC30A1 (ZnT1) knockout HeLa cell lysate

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SLC30A1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 1 and 3 bp deletion in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 1 and 3 bp deletion in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC30A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZnT1 also known as SLC30A1 is a critical protein responsible for the export of zinc ions out of cells. This transporter plays an important role in maintaining intracellular zinc homeostasis. The protein has a molecular mass of approximately 52 kDa. ZnT1 is expressed in a wide range of tissues including the brain liver and intestines indicating its significant role in various physiological processes. The protein is located in the cell plasma membrane which aligns with its function of mediating zinc efflux.
Biological function summary
ZnT1 is essential for regulating the cellular zinc levels and protecting against zinc toxicity. It does not form part of a larger complex; instead it operates as an individual unit to ensure that excess zinc does not accumulate to harmful levels within the cell. By controlling zinc efflux ZnT1 influences processes such as cell signaling and apoptosis which rely on zinc as a cofactor or structural component.
Pathways
Homeostasis of zinc ions in cells connects ZnT1 to the cellular zinc transport pathway and the metal ion transport pathway. ZnT1 coordinates with intracellular zinc-binding proteins like metallothioneins to modulate zinc availability and transportation within the cell. Proteins such as ZIP4 which facilitate zinc uptake operate in a complementary manner to ZnT1 balancing zinc influx and efflux to maintain cellular function.
Misregulation of ZnT1 has been linked to conditions such as cancer and neurodegenerative diseases. In cancer changes in zinc transport influence tumor growth and progression partly involving interactions with proteins like p53 a critical tumor suppressor modulated by zinc availability. In neurodegenerative diseases abnormal zinc levels can contribute to neuronal damage where ZnT1 interacts with Amyloid Beta plaques affecting the pathology of Alzheimer's disease.